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Image Search Results
Journal: Journal of the Royal Society Interface
Article Title: Combining in silico and in vitro models to inform cell seeding strategies in tissue engineering
doi: 10.1098/rsif.2019.0801
Figure Lengend Snippet: Different seeding cell strategies result in varying (a) cell density, (b) VEGF concentration and (c) oxygen concentration profiles along the length of the NRC geometry at different time points. In particular, non-uniform-seeded cell distributions (Sc = 3) result in more pronounced VEGF gradients in the centre of the constructs than uniform distributions (Sc = 1), although this varies over time and according the total number of cells seeded. Simulations suggest that the impact of the initial seeded cell distribution upon the VEGF and viable cell density distributions after 24 h varies according to the total number of cells seeded. Seeding 500 000 cells uniformly (Sc = 1) generates a steeper VEGF concentration gradient after 24 h than seeding more cells in the centre (Sc = 3); whereas the converse is true, when seeding 100 000 cells.
Article Snippet: Simulations of different cell densities and distributions over an
Techniques: Concentration Assay, Construct
Journal: Toxicological Sciences
Article Title: Cardiac Non-myocyte Cells Show Enhanced Pharmacological Function Suggestive of Contractile Maturity in Stem Cell Derived Cardiomyocyte Microtissues
doi: 10.1093/toxsci/kfw069
Figure Lengend Snippet: S100A1 regulates contraction in response to caffeine. CMEF microtissues were formed and incubated for 14 days prior to transfection with 10 nM nonsilencing siRNA (−ve control) and 10 nM S100A1 siRNA. A, RNA was extracted from microtissues and expression of GAPDH and S100A1 mRNA analysed by qRT-PCR (n = 3, ± SD). Contractile response recorded using the IonOptix cell geometry system under vehicle control (0.1% v/v DMSO) and 10 mM caffeine in (B) nonsilencing siRNA control CMEF microtissue and (C) S100A1 siRNA transfected CMEF microtissue (results from 1 experiment representative of 3 separate experiments).
Article Snippet: Contractile response recorded using the
Techniques: Incubation, Transfection, Control, Expressing, Quantitative RT-PCR